Anaplasma phagocytophilum – quality, Real-Time PCR (RUO/148)

Molecular diagnostics which is based on PCR methods (e.g. Real Time - PCR) is a sensitive test, whose primary limitation is difficulty in appropriate material selection. According to the recommendations the biopsy of skin lesions is the best material to make this test but analysis of systemic fluids such as: urine, blood or cerebrospinal fluid is also accepted. It should be remembered, however, that probability of bacteria detecting in systemic fluids is lower due to less frequent presence of this pathogen in the analyzed sample.

CB DNA test services include of all RUO (Research Use Only) tests which are designed only for the research purposes. Result of this tests is only for informational purposes and does not have a diagnostic test status.

The test consists in detecting the presence of DNA of Gram-negative bacteria Anaplasma phagocytophilum (previously known as Ehrlichia), which is an etiology factor of a disease called human granulocytic anaplasmosis. The bacteria gets to the blood stream together with secretion and saliva of a tick scavenging on human skin, perinatal infection is also possible as well as infection resulting from the exposure to infected blood. 

It has been examined that in Europe in case of approximately 30% of patients, infection with anaplasma coexists with tick-borne encephalitis or the Lyme disease. Symptoms of human granulocytic anaplasmosis are non-specific. The most frequent symptoms include: sudden fever, acute headache, shivers, ill-being and muscle pains. Less frequent include lack of appetite, vomiting, nausea, stomach aches, diarrhea or coughing.

In case of acute infections shocks are recorded, similar to septic shocks, myositis, including carditis, acute respiratory disorder syndrome,kidney disorder, intravascular clotting or a number of neurological syndromes. Prognosis gets worse with advanced age, chronic coexistant diseases (immunosuppression) as well as delayed application of appropriate antibiotic treatment.

Non-specific symptoms and total lack of symptoms in some cases creates substantial difficulties in proper disease identification. At the same time, if fast and accurate diagnosis is made, it facilitates total rehabilitation with the application of appropriate antibiotic treatment.

Test material:            whole blood (EDTA)

Transfer method:      temp. 4-8°C

Testing method:        Real-Time PCR

Delivery date:            up to 7 working days (for an extra fee /200zl/ the delivery date is up to 48 hours after delivery sample to the laboratory)